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Image Search Results
Journal: Nature
Article Title: Clonally expanded CD8 T cells characterize Amyotrophic Lateral Sclerosis 4
doi: 10.1038/s41586-022-04844-5
Figure Lengend Snippet: a, b, Peripheral blood cells from 8 month old WT and KI mice were analyzed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− SEM). d, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n=6 (left) and 2 independent experiments, n=4 (8 months) and n=6 (right) individual mice/group. SB=super bright.
Article Snippet: The following isotope conjugated anti-mouse antibodies were used at 1:100 dilution: 113 In CD45.1 (clone A20, eBioscience, Catalog # 14–0453-85), 115 In CD45.2 (clone 104, eBioscience, Catalog # 14–0454-85), 141 Pr Ly6G (clone 1A8, Fluidigm, Catalog # 3141008B), 142 Nd CD11c (clone N418, Biolegend, Catalog # 117302), 143 Nd TCRbeta (clone H57–597, Fluidigm, Catalog # 3143010B), 144 Nd CD24 (clone M1/69, Biolegend, Catalog # 101802),145 Nd CD69 (clone H1.2F3, Fluidigm, Catalog # 3145005B), 146 Nd F4/80 (clone BM8, Fluidigm), 147 Sm CD357/GITR (clone DTA-1, Biolegend), 148 Nd CD11b (clone M1/70, Biolegend, Catalog #), 149 Sm CD19 (clone 6D5, Fluidigm, Catalog #), 150 Nd IgD (clone 11–26c.2a, Biolegend, Catalog #), 151 Eu CD25 (clone 3C7, Fluidigm, Catalog # 3146008B), 152 Sm SiglecF (clone S17007L, Biolegend, Catalog # 155502), 153 Eu NKp46 (clone 29A1.4, Fluidigm, Catalog # 3153006B), 155 Gd CD73 (clone TY/11.8, Biolegend, Catalog # 127202), 156 Gd CD64 (clone X54–5/7.1, Biolegend, Catalog # 139302), 158 Gd CD117 (clone 2B8, Biolegend, Catalog # 105802), 159 Tb PD-1 (clone RMP1, Biolegend, Catalog #), 160 Gd CD62L (clone MEL-14, Fluidigm, Catalog # 109113),162 Dy Ly6C (clone HK1.4, Biolegend, Catalog # 128002),163 Dy anti-APC (Biolegend, Catalog # 408002),164 Dy Sca-1 (clone D7, Fluidigm, Catalog # 3164005B), 165 Ho anti-PE (Biolegend, Catalog # 408102), 166 Er KLRG1 (clone 2F1/KLRG1, Biolegend, Catalog # 93135),167 Er CXCR3 (clone CXCR3–173, Biolegend, Catalog # 126526), 168 Er CD8alpha (clone 53–6.7, Biolegend, Catalog # 100702), 169 Tm CD206 (clone C068C2, Fluidigm, Catalog # 3169021B),
Techniques: Mass Cytometry, Expressing, Flow Cytometry
Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
doi: 10.1007/978-1-0716-2553-8_10
Figure Lengend Snippet: Whole blood phosphoflow panel 1
Article Snippet: 142 Nd cCasp3 D3E9 Fluidigm 3142004A 143 Nd CD19 HIB19 Biolegend 302202 144 Nd pPLCg2 [Y759] K86-689.37 Fluidigm 3144015A 145 Nd CD4 RPA-T4 Fluidigm 3145001B 146 Nd IgD IA6-2 Fluidigm 3146005B 147 Sm CD20 2H7 Fluidigm 3147001B 148 Nd IgA Polyclonal Fluidigm 3148007B 149 Sm CD25 2A3 Fluidigm 3149010B 150 Nd pStat5 [Y694] 47 Fluidigm 3150005A 151 Eu CD123 6H6 Fluidigm 3151001B 153 Eu pStat1 [Y701] 4a Fluidigm 3153005A 154 Sm CD45 HI30 Fluidigm 3154001B 155Gd CD27 L128 Fluidigm 3155001B 156 Gd p38 [T180/Y182] D3F9 Fluidigm 3156002A 157 Gd CD24 ML-5 Biolegend 311102 158 Gd pStat3 [Y705] 4/P-Stat3 Fluidigm 3158005A 159Tb CD11c Bu15 Fluidigm 3159001B 160Gd CD14 M5E2 Fluidigm 3160001B 161 Dy CD141(BDCA-3) AD5-14H12 Miltenyi 130-090-694 162 Dy CD66b 80H3 Fluidigm 3162023B 163 Dy CD56 NCAM16.2 Fluidigm 3163007B 164 Dy IkBa L35A5 Fluidigm 3164004A 165 Ho pCREB [S133] 87G3 Fluidigm 3165009A 166 Er CD16 B73.1 Biolegend 360702 167 Er CD38 HIT2 Fluidigm 3167001B 168 Er CD8 SK1 Fluidigm 3168002B 169 Tm
Techniques:
Journal: Cell
Article Title: NKG2A blockade potentiates CD8 T-cell immunity induced by cancer vaccines
doi: 10.1016/j.cell.2018.10.028
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Blocking Assay, Purification, Virus, Recombinant, Staining, Transfection, Cell Stimulation, Adjuvant, Mutagenesis, Transgenic Assay, Marker, Sequencing, Expressing, Plasmid Preparation, Software, CRISPR
Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
doi: 10.1007/978-1-0716-2553-8_10
Figure Lengend Snippet: Whole blood phosphoflow panel 1
Article Snippet: 170 Er , CD3 , UCHT1 ,
Techniques: